Histology Guide

virtual microscopy laboratory

Chapter 4 - Muscle Tissue

Muscle tissue is composed of cells specialized for contraction. Muscle is classified into three types according to their structure and function:

Skeletal and cardiac muscle cells are called striated because they show an alternating series of bands. The repeating arrangement of their basic contractile unit, the sarcomere , produces these striations.

In all types of muscle, contraction is caused by the movement of myosin filaments along actin filaments .

The terms muscle cell and muscle fiber are synonymous.

Skeletal Muscle

Skeletal muscle fibers are long cylindrical, multinucleated, striated, and under voluntary control.

longitudinal and cross-section of skeletal muscle
Longitudinal and Cross-section
H&E
longitudinal section of skeletal muscle
Longitudinal Section
H&E
longitudinal section of skeletal muscle stained with phosphotungstic acid and hematoxylin
Longitudinal Section
Phosphotungstic Acid/Hematoxylin
longitudinal section of skeletal muscle
Longitudinal Section
H&E
cross-section of skeletal muscle
Cross-section
H&E

Individual skeletal muscle cells can be seen by teasing apart a muscle.

teased skeletal muscle cells
Skeletal Muscle Cells
H&E

Muscle Bone Junctions

Muscles connect to the skeletal framework to enable motion and provide stability. These connections transmit the forces generated by muscle fibers (cells) through two distinct mechanisms:

muscle and tendon junction
Muscle-Tendon Junction
H&E
muscle and tendon junction
Muscle-Tendon Junction
Azan
muscle insertion
Muscle Insertion
H&E

Cardiac Muscle

Cardiac muscle are short branching fibers, have a single, centrally located nucleus, show the same striations as skeletal muscle, and are under involuntary control.

cardiac muscle with intercalated discs stained with phosphotungstic acid and hematoxylin
Intercalated Discs
Phosphotungstic Acid/Hematoxylin

Purkinje fibers are modified cardiac muscle cells that convey electrical impulses that coordinate contraction of cardiac muscle.

purkinje fibers in heart
Purkinje Fibers
Periodic Acid-Schiff Reagent
purkinje fibers in heart
Purkinje Fibers
Phosphotungstic Acid/Hematoxylin

Smooth Muscle

Smooth muscle cells are spindle-shaped (fusiform), have a single, centrally located nucleus, and are under involuntary control. The uniform, nonstriated appearance gives rise to the name smooth muscle.

smooth muscle in blood vessels
Smooth Muscle
H&E
smooth muscle in small intestine
Smooth Muscle
H&E
smooth muscle in gallbladder
Smooth Muscle
H&E