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CHAPTER 4 - MUSCLE TISSUE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 074 Heart
TISSUE
Heart
Aortic Value and Cardiac Skeleton
(human)
STAINS
Phosphotungstic Acid/Hematoxylin (PTAH)
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZE
52,380 x 15,800 pixels
3.1 GB
FILE SIZE
8.1 GB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

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University of Minnesota
Department of Genetics, Cell Biology and Development
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321 Church St SE
Minneapolis, MN 55455

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MH 074 Heart

Purkinje Fibers
(Phosphotungstic Acid/Hematoxylin)

Purkinje fibers form the atrioventricular bundle (bundle of His), which is part of the specialized conduction system in the heart. This system generates the stimulus for the heart beat and conducts it to cardiac muscle

Purkinje fibers are modified muscle fibers. They have fewer myofibrils, but a higher content of glycogen. This allows their identification with phosphotungstic acid/hematoxylin (PTAH) stain which reveals cells with fewer myofibrils.

The is at the top of this specimen.

Beneath the endocardium are stained light gray. This lighter staining reflects the lower content of myofibrils in Purkinje cells.

Underneath the Purkinje cells, normal have a darker appearance because of their higher content of myofibrils.

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