Histology Guide

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MICROSCOPE SLIDE

SLIDE NAME

MH 044 Ground Bone

TISSUE

Tibia
(monkey)

PREPARATION

Ground Bone Section

IMAGE SIZE

27,160 x 28,865 pixels
2.9 GB

FILE SIZE

89 MB

OBJECTIVE

40x

PIXEL SIZE

0.3171 µm

SOURCE

Medical School
University of Minnesota
Minneapolis, MN

CONTACT

Questions or comments should be sent to
tcbrelje@gmail.com

This web site is owned and operated by:

T. Clark Brelje, Ph.D.

Faculty/Retired
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Version 6.0

Copyright © 2005-2024
T. Clark Brelje and Robert L. Sorenson
All rights reserved.

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MH 044 Ground Bone

Compact Bone

An alternative technique for examining bone is to saw bone into thin wafers and use abrasive surfaces to produce thin "ground" sections. Organic material is lost leaving only the mineral matrix of bone. The open spaces filled with air appear darker than the mineralized matrix.

- parallel layers of mineralized matrix on the surface of bone.
- - form the outer surface and receive nutrients from blood vessels in the periosteum.
- - form the inner surface and receive nutrients from blood vessels in the endosteum.
Osteons (, and ) - composed of concentric layers of mineralized matrix that surround a central canal. (Also see EM 217 Osteon.)
- Haversian Canals - circular opening at their center.
- Lacunae - small, lenticular spaces in the surrounding matrix.
Volkmann's Canals - openings that run perpendicular to Haversian canals connecting them with the surface ( and ) and each other ( and ). They carry blood vessels and nerve fibers into the Haversian system.

Many structures are easier to identify in sections of ground bone stained with dark dyes to reveal small, open spaces. See MH 202 Ground Bone (Schmorl's stain) and MH 233 Ground Bone (India ink).