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CHAPTER 9 - CARDIOVASCULAR SYSTEM
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAMES
MH 024 Mesentery
MH 025 Mesentery
MH 026 Mesentery
TISSUE
Mesentery
(human)
STAINS
Hematoxylin & Eosin (MH 024)
Verhoeff Stain (MH 025)
FIXATIVE
Zenker's Formaldehydes
IMAGE SIZES
96,060 x 37,797 pixels (MH 024)
13.5 GB
86,338 x 46,724 pixels (MH 025)
15 GB
FILE SIZES
6.08 GB (MH 024)
4.28 GB (MH 025)
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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T. Clark Brelje, Ph.D.

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 024-025 Mesentery

Click the thumbnail to show mesentery stained with hematoxylin & eosin.

Arterioles & Venules
(H&E)

Arterioles and venules are small diameter blood vessels that allow blood to flow into and out of capillary beds, respectively.

Compare H&E staining to that with Verhoeff.

  • Arteriole - small diameter blood vessel that branches out from arteries and leads to capillaries.
    • Their wall consists of endothelial cells, an internal elastic lamina apposed by one or two layers of smooth muscle, and a thin layer of collagen fibers. The inner elastic lamina is usually absent from smaller arterioles. The thickness of vessel wall is usually similar in size to the diameter of the lumen.
      • - one to two layers of smooth muscle and a remnant of an inner elastic lamina.
      • - adjacent arterioles.
      • - two layers of smooth muscle.
      • - nuclei of two endothelial cells and two smooth muscle cells are seen.
    • Arterioles are the primary site of vascular resistance.
  • Venules - small diameter blood vessel that allows blood to return from capillary beds to veins
    • Their wall is composed of an endothelial cell, one or two layers of smooth muscle, and very thin adventitia.

Examples of arterioles and venules (,,).

Click the thumbnail to show mesentery stained with Verhoeff stain.

Arteriole & Venule
(Verhoeff Stain)

Arterioles and venules are small diameter blood vessels that allow blood flow to and from capillary beds, respectively.

Verhoeff stain is used to stain the internal elastic lamina.

  • Arteriole - small diameter blood vessel that branches out from arteries and leads to capillaries.
    • Their wall consists of endothelial cells, an internal elastic lamina apposed by one or two layers of smooth muscle, and a thin layer of collagen fibers. The inner elastic lamina is usually absent from smaller arterioles. The thickness of vessel wall is usually similar in size to the diameter of the lumen.
      • - one to two layers of smooth muscle and a remnant of an internal elastic lamina.
      • - one to two layers of smooth muscle and a remnant of an internal elastic lamina.
      • - two layers of smooth muscle and an internal elastic lamina.
    • Arterioles are the primary site of vascular resistance.
  • Venules - small diameter blood vessel that allows blood to return from capillary beds to veins
    • Their wall is composed of an endothelial cell, one or two layers of smooth muscle, and very thin adventitia.

Examples of arterioles and venules (,,).

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