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CHAPTER 8 - HEMATOPOIESIS
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 034a Bone Marrow Smear
TISSUE
Bone Marrow Smear
(human)
STAIN
Wright's Stain
(mixture of methylene blue,
azure II, and eosin)
FIXATIVE
Air Dry
Methanol
IMAGE SIZE
116,436 x 83,634 pixels
36 GB
FILE SIZE
2.6 GB
OBJECTIVE
40x
PIXEL SIZE
0.2555 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 034a Bone Marrow Smear

Bone Marrow Smear

Bone marrow smears are made by placing a drop of bone marrow on one end of a slide, and using a spreader slide to disperse the marrow from left to right.

  • - left side of the slide with the largest number of cells. The tight packing of cells distorts their .
  • - region where the cells are spaced far enough apart to be differentiated and counted. The is best in this zone.
    • (Metaphase) - many cell types in bone marrow have high rates of cell division.
  • - right side of the slide with the lowest number of cells. The of the cells is typically poor.

This scan was obtained with a 40x dry objective. Except for identifying the zone of best morphology, individual cells need to be viewed at higher magnification to examine their morphology.

See MH 034ahr Bone Marrow Smear for an image of the feather edge acquired with a 60x oil immersion objective.

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