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In 1898, Camillo Golgi described an "internal reticular apparatus" in cells stained using Golgi's method (silver stain). Many years later, electron microscopy confirmed the discovery of this organelle.
It’s relatively easy to see the Golgi apparatus in the cells lining the epididymis, the organ of the male reproductive tract in which sperm mature. Examine the septa composed of two layers of cells facing adjacent cross sections of the duct separated by a small amount of connective tissue.
The Golgi apparatus is located between the nucleus and the apical pole of the cell (i.e., supranuclear). It’s reticular structure is stained black.
These cells are polarized with their basal surface resting on the underlying connective tissue while the apical surface faces the lumen.
Pancreas
Most of the pancreas is composed of acinar cells. They are polarized cells clustered around a central lumen that is the entrance to a duct (i.e., an acinus). Unfortunately, the staining of the pancreas is highly variable (either under or over stained). Search for a region in which only the Golgi apparatus is stained.
The Golgi apparatus is located between the nucleus and the apical pole of the cell (i.e., supranuclear). It’s reticular structure is stained black.
These cells are polarized with their basal surface resting on the underlying connective tissue while the apical surface faces the lumen.