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CHAPTER 3 - CONNECTIVE TISSUE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 008 Pancreas
TISSUE
Pancreas
(rat)
STAIN
Aldehyde Fuchsin
FIXATIVE
Bouin's Solution
IMAGE SIZE
50,440 x 31,786 pixels
6 GB
FILE SIZE
441 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
Department of Genetics, Cell Biology and Development
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MH 008 Pancreas

Mast Cells
(Aldehyde Fuchsin)

Mast cells are involved in inflammation and immune responses. They release histamine, heparin and other inflammatory mediators.

Aldehyde fuchsin produces violet staining of mast cell granules, elastic fibers, and islets of Langerhans.

  • Mast Cells - oval-shaped cells with intensely stained purple cytoplasm.
    • - individual cells scattered in the connective tissue around blood vessels. Compare the to adjacent adipocytes.
    • - individual cells in connective tissue around blood vessels.
  • - stain purple in blood vessels.
  • - numerous islands ("islets") of beta cells with purple stained insulin-containing secretion granules. These endocrine cells are unrelated to mast cells.

Mast cells in connective tissue can be identified by their abundant, large secretion granules by transmission electron microscopy. See EM 108 Mast Cells and EM 110 Mast Cell.

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