Skip To Main Content
CHAPTER 6 - NERVOUS TISSUE
Histology Guide
application menu
  • HOME
  • SLIDE BOX
  • CHAPTER 6 - NERVOUS TISSUE
  • INDEX
  • SEARCH
  • TERMS OF USE
  • HELP

MICROSCOPE SLIDE

SLIDE NAME
MHS 285-286 Spinal Ganglion
TISSUE
Spinal Ganglion (rat)
STAIN
Toluidine Blue (MHS 285) / Eosin
Myelin Stain (MHS 286)
IMAGE SIZE
16,176 x 15,229 pixels
918 MB (MHS 285)
19,087 x 15,992 pixels
1.14 GB (MHS 286)
FILE SIZE
37 MB (MHS 285)
47 MB (MHS 286)
OBJECTIVE
40x
PIXEL SIZE
0.2508 µm
SOURCE
Zoology Department
University of Minnesota
Minneapolis, MN

SETTINGS

Version 8.0


Display mode

Viewer options


Move Between WayPoints:



Description

Font size

CONTACT US

Questions or comments should be sent to
tcbrelje@gmail.com

This web site is owned and operated by:

T. Clark Brelje, Ph.D.

Faculty/Retired
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

See Terms of Use for more information.

HELP

See HELP for more extensive information.

Get the User Guide v1.1 to discover new features that can enhance your use of this platform.

Each slide is shown with additional information to its right. The image can be changed using any combination of the following commands.

Sidebar

  • Links: Click to navigate to a specific region
  • Images: Click to show this view
  • Toolbar: Use controls to adjust magnification and pan the image

Mouse

  • Zoom In: Click left button
  • Zoom Out: Double-click left button
  • Pan/Move: Click and drag the image

Keyboard

  • Zoom In: ‘A’ key
  • Zoom Out: ‘Z’ key
  • Pan/Move: Arrow keys (Up, Down, Left, Right)
  • Reset View: ESC key (fit-to-screen view)

Touch

  • Tap: Zoom in on a specific area
  • Double-tap: Zoom out from the current view
  • Drag: Pan the image

SHARE

A link to a virtual slide can be saved for later viewing in different ways.

Clipboard

The address of this view has been copied to your clipboard. This link can be pasted in any other program.

Bookmark

A bookmark link can be created using the bookmark function (Ctrl-D for Windows or Cmd-D for Mac) of your browser. Choose a name for the bookmark and select the folder in which you want it saved.

MHS 285-286 Spinal Ganglion

Click the thumbnail to show dorsal root ganglion stained with toluidine blue.

Dorsal Root Ganglion
(Toluidine)

Toluidine blue is a basic dye that binds nucleic acids, but preferentially stains RNA.

is an aggregation of sensory neurons (pseudo-unipolar) located on each dorsal spinal root.

  • - large, nerve cell bodies with centrally located nuclei
    • Nucleolus - intensely stained because it contains negatively charged RNA involved in ribosome assembly
    • Nissl (chromophil) Substance - contains negatively charged RNA found in free ribosomes and ribosomes bound to endoplasmic reticulum (i.e., RER)
      • Only found in cell body and dendrites
      • Abundant chromophil substance suggests these neurons synthesize large amounts of protein
  • (or capsule cells)- small, glial cells at the periphery of nerve cell bodies
  • - non-myelinated and myelinated axons of different diameters
    • - unstained because lipids of the myelin sheaths were extracted during staining (appears foamy)
    • - fragments of dense irregular connective tissue that surround nerves

Click the thumbnail to show dorsal root ganglion stained with myelin stain.

Dorsal Root Ganglion
(Weigert's Stain)

Weigert's stan involves preserving myelin lipids with potassium dichromate and staining with hematoxylin. Myelin sheaths appear black, providing a useful technique for visualizing fiber pathways.

is an aggregation of sensory neurons (pseudo-unipolar) located on each spinal root.

  • - cell bodies are non-specifically stained to varying degrees from yellow to black
    • Because of their small size relative the cell bodies, the axon exiting the cell bodies is seen infrequently (,,).
  • Satellite Cells (or capsule cells) - unstained (light yellow)
  • - myelinated axons of different diameters
    • The between myelinated fibers are occupied by unstained, non-myelinated fibers (light yellow - orange)
© 2005-2026. T. Clark Brelje and Robert L. Sorenson