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CHAPTER 1 - THE CELL
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MHS 268 Pancreas
TISSUE
Pancreas
(rat)
STAIN
Hematoxylin & Eosin
FIXATIVE
4% Formaldehyde
IMAGE SIZE
37,182 x 33,065 pixels
3.43 GB
FILE SIZE
2.54 GB
OBJECTIVE
40x
PIXEL SIZE
0.2494 µm
SOURCE
T. Clark Brelje and Robert L. Sorenson
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MHS 268 Pancreas

Biological material is inherently of low contrast and provides little to see in a standard bright field microscope unless treated with a histological stain.

What dye(s) were used to stain this ? [ + ]

Hematoxylin and eosin (H&E) are the most commonly used dyes in histology and pathology.

H&E stain components found in the nucleus, cytoplasm, and extracellular matrix.

The nuclei and cytoplasm of individual cells are easily identified. This reflects the complementary binding affinities of these dyes for many cellular components.

Which dye is positively charged (i.e., a basic dye)?


What are structures stained with basic dyes called? [ + ]

Basophilic

What cellular component(s) of a cell have a high affinity for basic dyes? [ + ]

Hematoxylin binds negatively-charged phosphate groups of nucleic acids (DNA and RNA)
  • Nuclei are intensely stained
  • Cytoplasm of cells are lightly stained
Which dye is negatively charged (i.e., an acidic dye)?

What are structures stained with acidic dyes called? [ + ]

Acidophilic (or eosinophilic)

What cellular component(s) of a cell have a high affinity for acidophilic dyes? [ + ]

Eosin binds to positively-charged amino groups of proteins (mostly side chains of lysine and arginine)
  • Nuclei are lightly stained
  • Cytoplasm of most cells is more intensely stained (secretion granules can often be seen in the cytoplasm of secretory cells)
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