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A nerve is a bundle of nerve fascicles surrounded by a connective tissue sheath (epineurium). This is easily seen in this specimen stained with Masson's trichrome.
Connective tissue - collagen fibers stain blue.
Epineurium - dense irregular connective tissue surrounding the entire nerve.
Perineurium - connective tissue surrounding a bundle of nerve fibers (fascicle). It consists of two parts:
Fibrous Layer - an outer layer of connective tissue (blue) that is similar to the endoneurium.
Cellular Layer - inner cellular layer (3 to 8 cells thick) of flattened fibroblasts (blue/gray cells).
