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CHAPTER 5 - CARTILAGE AND BONE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 043 Cancellous and Compact Bone
TISSUE
Parietal Skull
(human)
PREPARATION
Decalcified Bone
STAIN
Hematoxylin & Eosin
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZE
96,030 x 31,285 pixels
11 GB
FILE SIZE
230 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 043 Cancellous and Compact Bone

Compact and Spongy Bone

Bone must be decalcified (by exposure to strong acids) so it can be cut into thin sections. The remaining material is mostly collagen.

This slide contains two samples:

  • - partial cross-section from the end of a long bone found in the extremities.
  • - cross-section of a bone from the skull cap (calvarium).

Most bones contain both compact and spongy bone.

Two structural arrangements of bone tissue are seen:

  • Compact Bone - a dense layer that forms the outer shell or cortex of bones.
    • - forms the outer circular surface.
    • Flat Bone - forms the and flat surfaces.
  • Spongy (Cancellous or Trabecular) bone - anastomosing network of spicules found in the interior of bones.
    • - often found near the ends of long bones.
    • - fills the narrow interior of flat bones.

A three-dimensional view of compact and spongy bone is seen in EM 215 Tibia by scanning electron microscopy.

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