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CHAPTER 5 - CARTILAGE AND BONE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 038 Epiglottis
MH 039 Epiglottis
TISSUE
Epiglottis
(human)
STAIN
Hematoxylin & Eosin (MH 038)
Verhoeff Stain (MH 039)
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZES
90,210 x 32,032 pixels (MH 038)
10.8 GB
82,560 x 27,525 pixels (MH 039)
8.5 GB
FILE SIZES
368 MB (MH 038)
348 MB (MH 039)
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Faculty/Retired
University of Minnesota
Department of Genetics, Cell Biology and Development
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 038-039 Epiglottis

Click the thumbnail to show epiglottis stained with hematoxylin & eosin.

Epiglottis in cross section.

What dye(s) were used to stain this ? [ + ]

Hematoxylin and eosin (H&E)

What type of is present? [ + ]

Hyaline or elastic cartilage – both have similar appearances when stained by H&E (elastic fibers are unstained)
Tissue section must be stained for elastin to convincingly identify elastic cartilage

Click the thumbnail to show epiglottis stained with Verhoeff stain.

Epiglottis in cross section.

What dye(s) were used to stain this ? [ + ]

Verhoeff stain

What type of is stained intensely black? [ + ]

Elastic fibers in the cartilage matrix

What type of cartilage is present in the epiglottis? [ + ]

Elastic cartilage

How do the physical properties of elastic cartilage differ from hyaline cartilage? [ + ]

Abundance of elastic fibers allows flexibility and returns to its shape

Where can elastic cartilage be found? [ + ]

  • External ear
  • Epiglottis
  • Larynx
  • Auditory tubes
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