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Click the thumbnail to show this specimen stained with hematoxylin & eosin.
Of the fibrous components in connective tissue, hematoxylin & eosin (H&E) stains both collagen and muscle.
Examine the staining of a blood vessel in this section of mesentery:
Collagen - the outer wall is composed of collagen fibers stained pink/red
Elastic Fibers - the crenulated structure in the inner wall is composed of elastic fibers that are unstained (or light pink)
Reticular Fibers - unstained
Muscle - the middle region of the wall is composed of smooth muscle stained pink
Although both collagen and muscle are stained pink/red by H&E, they can still be distinguished from each other based on their morphology and location.
H&E
Azan
Verhoeff
Collagen
pink/red
blue
red
Elastic Fiber
colorless
colorless
black
Reticular Fiber
colorless
colorless
colorless
Muscle
pink
pink
colorless
Azan
Click the thumbnail to show this specimen stained with azan.
Of the fibrous components in connective tissue, azan is used to distinguish collagen from muscle.
Examine the staining of a blood vessel in this section of mesentery:
Collagen - the outer wall is composed of collagen fibers stained blue
Elastic Fibers - the crenulated structure in the inner wall is composed of elastic fibers that are unstained (or red)
Reticular Fibers - unstained
Muscle - the middle region of the wall is composed of smooth muscle stained pink/red
Unlike H&E, collagen (blue) and muscle (red) are stained different colors by azan.
H&E
Azan
Verhoeff
Collagen
pink/red
blue
red
Elastic Fiber
colorless
colorless
black
Reticular Fiber
colorless
colorless
colorless
Muscle
pink
pink
colorless
Verhoeff Stain
Click the thumbnail to show this specimen stained with Verhoeff stain.
Of the fibrous components in connective tissue, Verhoeff stain is used to identify elastic fibers.
Examine the staining of a blood vessel in this section of mesentery:
Collagen - the outer wall is composed of collagen fibers stained red
Elastic Fibers - the crenulated structure in the inner wall is composed of elastic fibers stained black. Another band of elastic fibers is found between the layers of muscle and collagen.
Reticular Fibers - unstained
Muscle - the middle region of the wall is composed of smooth muscle that is unstained (or brown)
Unlike H&E and azan, elastic fibers (black) are stained by Verhoeff.
