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CHAPTER 5 - CARTILAGE AND BONE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 020b Pig Snout Fetal
TISSUE
Pig Snout
(fetal)
STAIN
Hematoxylin & Eosin
FIXATIVE
10% Formaldehyde
IMAGE SIZE
34,920 x 15,866 pixels
2.1 GB
FILE SIZE
145 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

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University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 020b Pig Snout Fetal

Intramembranous Bone Formation

This section contains many areas of intramembranous bone formation. Bone is formed by the differentiation of mesenchymal cells into osteoblasts.

Examine the with newly formed, basophilic bone:

  • - cover the surface of bone spicules. Their very is from the synthesis of proteins in osteoid (mostly type I collagen).
  • - unmineralized, organic portion (type I collagen and ground substance) of bone matrix. Narrow, light-pink region between osteoblasts and the bone spicule.
  • - osteoblasts trapped inside lacunae of the bone.

The spicules are examples of . They contain randomly arranged type I collagen fibers within a basophilic, mineralized osteoid.

The spicules fuse with each other and from trabeculae. These provide the general shape of the developing bone.

There is little evidence of osteons found in mature (or secondary) bone. A few developing osteons (,) can be recognized by blood vessels at the center of an area surrounded by osteoblasts.

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