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CHAPTER 1 - THE CELL
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 003 Toluidine Blue
TISSUE
Pancreas (rat)
Lymph Node (rat)
Cerebellum (rat)
Liver (rat)
STAIN
Toluidine Blue
FIXATIVE
Bouin's Solution
IMAGE SIZE
93,145 x 66,275 pixels
23 GB
FILE SIZE
5.39 GB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
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321 Church St SE
Minneapolis, MN 55455

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MH 003 Toluidine Blue

What dye(s) were used to stain these sections? [ + ]

Toluidine blue

Examine the large in the cerebellum.

What structures stain with this dye?


What type of nucleic acids stains intensely with this dye? [ + ]

RNA

What component of the nucleus stains intensely with this dye? [ + ]

Nucleolus

What process occurs in this structure? [ + ]

Ribosome assembly

What is the granular material in the cytoplasm named in neurons? [ + ]

Nissl substance - contains negatively charged RNA found in free ribosomes and ribosomes bound to endoplasmic reticulum (i.e., RER)

What does its presence suggest about these cells? [ + ]

They synthesize large amounts of proteins

Describe the staining of in the pancreas: [ + ]

  • Apical portion contains unstained secretion granules
  • Basal portion of cells is intensely stained (i.e., rough endoplasmic reticulum; RER)
  • Nuclei are unstained except for the nucleolus

Why is the basal region of these cells intensely stained? [ + ]

It contains large amounts of negatively charged RNA found in ribosomes bound to endoplasmic reticulum (i.e., RER) and free ribosomes

What does its presence suggest about these cells? [ + ]

They synthesize large amounts of proteins

Describe the staining of in the lymph node: [ + ]

Their nuclei are stained and contain mostly heterochromatin

What does the small amount of unstained cytoplasm suggest about these cells? [ + ]

They are not synthesizing much protein

What is staining the same color as the dye known as? [ + ]

Orthochromatic – stains the same color of the dye (i.e., blue)

Describe the staining of : [ + ]

Their secretion granules are stained dark purple and obscure the nuclei

When staining is a different color than dye it is described as? [ + ]

Metachromasia – stains a different color than the dye (i.e., dark purple)

Why does this occur? [ + ]

Intense staining due to the high concentration of negative charges found in heparin in these granules
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