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CHAPTER 1 - THE CELL
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 002 Cells and Tissues
TISSUE
Blood Cells (human)
Trachea (monkey)
Small Intestine (monkey)
STAIN
Hematoxylin & Eosin
IMAGE SIZE
150,720 x 81,008 pixels
45.5 GB
FILE SIZE
958 MB
OBJECTIVE
40x
PIXEL SIZE
0.3666 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

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University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 002 Cells and Tissues

Red blood cells can be used as cellular rulers to determine the size of other structures.

What is the average diameter of ? [ + ]

7.5 µm

Describe the staining of :


Do red blood cells have nuclei?


Blood cells with nuclei are known as white blood cells (,,).

Do the nuclei of white blood cells vary in shape?


What is the range of diameters of white blood cells? [ + ]

9 to 25 µm

Trachea contains examples of basophilia and acidophilia.

Describe the staining of the cartilage:


Why does hematoxylin stain cartilage? [ + ]

Besides nucleic acids (DNA and RNA), it also stains other negatively charged molecules. For example, the sulfated polysaccharides found in the extracellular matrix of cartilage.

Describe the staining of the extracellular protein, collagen:


Why does eosin stain collagen fibers? [ + ]

Collagen fibers are aggregates of long proteins with many positively-charged amino acids
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