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CHAPTER 19 - FEMALE REPRODUCTIVE SYSTEM
Histology Guide
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MICROGRAPH

NAME
EM 325 Secondary Follcile
TISSUE
Ovary
IMAGE SIZE
10,792 x 7,189 pixels
222 MB
FILE SIZES
46,051 KB (grayscale)
40,755 KB (color)
MAGNIFICATION
Unknown
PIXEL SIZE
12.80 nm
SOURCE
P. Bagavandoss
Department of Anatomy
University of Michigan
Ann Arbor, Michigan

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EM 325 Primary Follicle

Secondary Follicle

A secondary (antral) follicle contains an oocyte surrounded by a layer of glycoproteins (zona pellucida) and cumulus cells (cumulus oophorus). The later form a hillock that attaches it the granulosa cells that surround that fluid-filled antrum.

Transmission electron micrograph (TEM) of the innermost cumulus layer (corona radiata) that sends processes through the zona pellucida to form contacts with the oocyte.

  • (yellow) - numerous irregular microvilli extend into the zona pellucida
  • (purple) - extracellular coat of glycoproteins that forms an extracellular coat around the oocyte
  • (green) - cuboidal cells with projections that extend into the zona pellucida. Some of which contact the plasma membrane of the oocyte
    • Extensive gap junctions develop between adjacent granulosa cells
    • Important for binding of spermatozoa
    • Induces the acrosome reaction to release enzymes that enable a spermatozoon to penetrate the zona pellucida

Subcellular Structures

  • Nucleus (blue) / Nuclear Envelope (purple)
  • Mitochondria (red)

Courtesy of P. Bagavandoss, Department of Anatomy, University of Michigan, Ann Arbor, Michigan.

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